UMI family sizes (Simplex reads)

Understanding the frequency of UMI families of different read counts

Theoretical Method

In this plot we investigate the number of families of each discrete size for simplex reads, which consist of 3 or more read pairs from one of the two strands.

Technical Methods

• Tools Used:

  • Marianas

  • make_umi_qc_tables.sh

• Input

  • collapsed_R1_.fastq

  • collapsed_R2_.fastq

  • MSK-ACCESS-v1_0-A-on-target-positions.txt

  • MSK-ACCESS-v1_0-B-on-target-positions.txt

• Output

  • family-sizes.txt

Interpretations

This graph begins at family sizes of 3, for which the largest number of families should occur, and drops off after that.