Average Coverage, Sample Level, Pool B Targets
Detailed view of coverage values for each sample, grouped by UMI family type
Theoretical Method
Similarly to the Pool A Targets, coverage is calculated for each UMI family type, over the Pool B genomic bait regions. These coverage values are lower for cfDNA samples (which use a 50:1 pool ratio) but should be comparable for buffy coat samples (which use a 1:1 pool ratio).
Technical Methods
Tool Used:
Marianas
Waltz CountReads
aggregate_bam_metrics.sh
tables_module.py
plots_module.r
Input
4 bams per sample (Standard, U, S, D)
Output
sample_id-intervals.txt (sample level, included for all 4 bam types)
waltz-coverage.txt (aggregated across samples, for a single bam type)
coverage_agg.txt (aggregated across all samples, all bam types, pools A / B)
Interpretations Aim is to have high coverage, and as much duplex “saturation” as possible. See title page for specific pass / fail criteria.
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