Access Quality Control (v1)
  • Introduction
  • Meta information per sample
  • Raw read-pair counts (standard BAM)
  • On Target Coverage
  • Fraction of reads mapping to the human genome
  • “On Bait” reads localized to ACCESS panel
  • Coverage vs GC content
  • Insert Size Distribution
  • Distribution of ACCESS panel A coverage values
  • Average Coverage, Sample Level, Pool A Targets
  • UMI Family types Composition (Pool A)
  • Average Coverage, Sample Level, Pool B Targets
  • UMI Family types Composition (Pool B)
  • Base Quality Recalibration Scores
  • UMI family sizes (Simplex reads)
  • UMI family sizes (Duplex reads)
  • Sample Level Noise
  • Noise by Substitution Type
  • Contributing Sites for Noise
  • Hotspots In Normals
  • Sample mix-up
  • (Un)expected (Mis)matches Tables
  • Major Contamination
  • Minor Contamination
  • Duplex Minor Contamination
  • Sex Mismatch
  • FAQ
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Waltz Metrics Files

  • .covered-regions

    • chr

    • start

    • end

    • length

    • average coverage in the contiguous region

    • total coverage in the contiguous region

  • .read-counts

    • bam file name

    • total reads

    • unmapped reads

    • total mapped reads

    • unique mapped reads

    • duplicate fraction

    • total on-target reads

    • unique on-target reads

    • total on-target rate

    • unique on-target rate

  • .fragment-sizes

    • fragment-size

    • total frequency

    • unique frequency

  • -pileup-without-duplicates.txt

    • similar to above but only unique fragments are counted

  • -intervals.txt Header

    • chr

    • start

    • end

    • interval name

    • interval length

    • peak coverage

    • average coverage

    • GC fraction

    • number of fragments mapped

  • -intervals-without-duplicates.txt

    • similar to above but only unique fragments are considered

After aggregate_bam_metrics.sh (aggregate across samples):

  • waltz-coverage.txt - per sample coverage calculated across chosen genomic intervals

  • fragment-sizes.txt - fragment size distributions for all samples

PreviousSex Mismatch

Last updated 5 years ago

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