Meta information per sample

Overview of library and coverage information for the current set of samples run through ACCESS assay

Technical Method

• Tool Used: plots_module.r

• Input:

  • title_file.txt

  • coverage_agg.txt

  • average_coverage_across_exon_targets_duplex_A.txt

• Output: N/A

Interpretation

• Library input should be ~5-20ng for ctDNA, ~200ng for buffy coats (or the maximum amount available if these thresholds can’t be met)

• Capture input should be ~500ng or maximum available after library generation

• Expected range of coverage values:

  • • Raw coverage A panel:

      • ctDNA: ~ 15000x-20000x

      • Buffy Coat: ~ 500x-1000x

    • Raw coverage B panel:

      • ctDNA: ~ 1000x-1,500x

      • Buffy Coat: ~ 500x-1000x

    • Duplex coverage A panel:

      • ctDNA: ~ 500x-2000x

      • Buffy Coat: ~ 10x-50x

Note: Samples that don’t meet the library input criteria will have lower coverage