Nucleo Quality Control
  • 🌸Nucleo Quality Control
    • Workflow description
    • Requirements
    • Installation and Usage
    • Workflow inputs
      • Input for nucleo_qc.cwl
      • Input for nucleo_aggregate_visualize.cwl
    • Workflow outputs
    • ☀️Interpretation - MultiQC
      • Summary QC metrics
      • Coverage vs GC content
      • Capture Metrics
      • Insert Size
      • Target coverage distribution
      • Duplex Metrics
        • Duplex family sizes
        • Simplex family sizes
        • Duplex yield metrics
      • Mean Base Quality
      • Duplex Noise Metrics
      • Contamination
        • Hotspot in Normals
        • Major contamination
        • Minor contamination
        • Contributing sites
      • Fingerprinting
      • QC thresholds
  • 🇬🇷Interpretation - Athena Coverage report
    • Installation and running
    • Interpretation coverage report
      • Report details
      • Summary
      • Exons with <100% coverage at 500x
      • Per gene coverage summary
      • Per exon coverage
      • Coverage of Known Variants
      • Coverage per chromosome
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  1. Interpretation - Athena Coverage report

Installation and running

PreviousInterpretation - Athena Coverage reportNextInterpretation coverage report

Last updated 2 years ago

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The athena tool is made of 3 parts; 1) annotate the bed file 2) generate statistic files 3) generate the coverage report. This can be run independently or together with a single workflow:

Run independently:

  1. Annotate each region of the bed file with the gene, exon and per base coverage data using

  2. Generate per exon and per gene statistics using

  3. Generate HTML coverage report with

Run three steps with a single workflow:

  • Run all 3 steps above using a single workflow using

🇬🇷
https://github.com/msk-access/cwl-commandlinetools/blob/develop/athena/1.4.2/annotate_bed/annotate_bed.cwl
https://github.com/msk-access/cwl-commandlinetools/blob/develop/athena/1.4.2/coverage_stats_single/coverage_stats_single.cwl
https://github.com/msk-access/cwl-commandlinetools/tree/develop/athena/1.4.2/coverage_report_single
https://github.com/msk-access/cwl_subworkflows/blob/develop/athena_report/athena_report.cwl