Capture metrics

Validating the efficacy of the Pool A and Pool B bait sets.

Introduction

There are several sections displaying bait set capture efficiency. Each section corresponds to a separate BAM type and bait set combination. The tool used to produce the metrics is GATK-CollectHsMetrics. By default, only the mean bait coverage, mean target coverage, and % Usable bases on-target are displayed. However, there are many more metrics that can be toggled to display by clicking on the Configure Columns button.

Example MultiQC report showing insert size distribution for 20 samples (10 plasma and 10 buffy coat samples).

Methods

Tool used: GATK-CollectHsMetrics BAM type: (1) Uncollapsed BAM, (1) Collapsed BAM, (1) Duplex BAM, and (4) Simplex BAM Regions: Pool A and Pool B

Interpretation

The aim is to have high coverage across Pool A and Pool B panels.