Capture metrics

Validating the efficacy of the Pool A and Pool B bait sets.

Introduction

There are several sections displaying bait set capture efficiency. Each section corresponds to a separate BAM type and bait set combination. The tool used to produce the metrics is GATK-CollectHsMetrics. By default, only the mean bait coverage, mean target coverage, and % Usable bases on-target are displayed. However, there are many more metrics that can be toggled to display by clicking on the Configure Columns button.

Methods

Tool used: GATK-CollectHsMetrics BAM type: (1) Uncollapsed BAM, (1) Collapsed BAM, (1) Duplex BAM, and (4) Simplex BAM Regions: Pool A and Pool B

Interpretation

The aim is to have high coverage across Pool A and Pool B panels.

Last updated