# Introduction Marianas is a java program used for generating accurate base calls from high-throughput sequencing data using Unique Molecular Identifier technology. Since Marianas uses serial access over the entire bam file, it is highly efficient and processes all reads irrespective of target regions. Typically, Marianas collapsing for an MSK ACCESS sample would finish within 20 minutes, producing collapsed fastq files and QC metrics related to the collapsing process. ![Overview of the Marianas processing steps](https://461638448-files.gitbook.io/~/files/v0/b/gitbook-legacy-files/o/assets%2F-M6GVE_FJ6Uok3BwY66L%2F-M7JfEQA7k1mBlmdKymW%2F-M7JgMA5jxN84H420rxf%2FScreen%20Shot%202020-05-14%20at%203.37.49%20PM.png?alt=media\&token=464ec26c-d9c2-4ea1-b107-c96c799d153c) This program was developed by [Juber Patel](https://github.com/juberpatel) in the [Center for Molecular Oncology](https://www.mskcc.org/research-programs/molecular-oncology) at [Memorial Sloan Kettering Cancer Center](https://www.mskcc.org). The source code for this tool can be found on the [Github page](https://github.com/mskcc/Marianas). Documentation has been compiled by [Juber Patel](https://github.com/juberpatel), [Ian Johnson](https://github.com/ionox0) with contributions from all members of [CMO Innovation lab](https://www.mskcc.org/research-programs/molecular-oncology/innovation-laboratory), [Berger Lab](https://www.mskcc.org/research-areas/labs/michael-berger), and [Clinical Bioinformatics groups](https://mskcc-clinbx.github.io/team/). --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://cmo-ci.gitbook.io/marianas/master.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.