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description: Validating the efficacy of the Pool A and Pool B bait sets.
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# Capture metrics

## Introduction

There are several sections displaying bait set capture efficiency. Each section corresponds to a separate BAM type and bait set combination. The tool used to produce the metrics is GATK-CollectHsMetrics. By default, only the mean bait coverage, mean target coverage, and % Usable bases on-target are displayed. However, there are many more metrics that can be toggled to display by clicking on the `Configure Columns` button.

![Example MultiQC report showing insert size distribution for 20 samples (10 plasma and 10 buffy coat samples).](https://3106615528-files.gitbook.io/~/files/v0/b/gitbook-legacy-files/o/assets%2F-MbqIfOAQqfG2kMfi2uM%2Fsync%2Fbd1cbaa0dad3d23a897e519764a167e364835852.png?generation=1624554771157328\&alt=media)

## Methods

**Tool used:** [GATK-CollectHsMetrics](https://gatk.broadinstitute.org/hc/en-us/articles/360036856051-CollectHsMetrics-Picard-)\
&#x20;**BAM type:** (1) Uncollapsed BAM, (1) Collapsed BAM, (1) Duplex BAM, and (4) Simplex BAM\
&#x20;**Regions:** Pool A and Pool B

## Interpretation

The aim is to have high coverage across Pool A and Pool B panels.
