UMI family sizes (Simplex reads)

Theoretical Method

In this plot we investigate the number of families of each discrete size for simplex reads, which consist of 3 or more read pairs from one of the two strands.

Technical Methods

• Tools Used:

  • Marianas

  • make_umi_qc_tables.sh

• Input

  • collapsed_R1_.fastq

  • collapsed_R2_.fastq

• Output

  • family-sizes.txt

Interpretations

This graph begins at family sizes of 3, for which the largest number of families should occur, and drops off after that.