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Meta information per sample

Overview of library and coverage information for the current set of samples run through ACCESS assay

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Technical Method

  • Tool Used: plots_module.r

  • Input:

    • title_file.txt

    • coverage_agg.txt

    • average_coverage_across_exon_targets_duplex_A.txt

  • Output: N/A

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Interpretation

  • Library input should be ~5-20ng for ctDNA, ~200ng for buffy coats (or the maximum amount available if these thresholds can’t be met)

  • Capture input should be ~500ng or maximum available after library generation

  • Expected range of coverage values:

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Note: Samples that don’t meet the library input criteria will have lower coverage

  • Raw coverage A panel:

    • ctDNA: ~ 15000x-20000x

    • Buffy Coat: ~ 500x-1000x

  • Raw coverage B panel:

    • ctDNA: ~ 1000x-1,500x

    • Buffy Coat: ~ 500x-1000x

  • Duplex coverage A panel:

    • ctDNA: ~ 500x-2000x

    • Buffy Coat: ~ 10x-50x