---
description: >-
  Overview of library and coverage information for the current set of samples
  run through ACCESS assay
---

# Meta information per sample

![](https://2763969089-files.gitbook.io/~/files/v0/b/gitbook-legacy-files/o/assets%2F-M52gq1rRSDQOKMQGEuR%2F-M9Z39Nd7c6cJRknLQBm%2F-M9Z55bruZykVtLxd0It%2FScreen%20Shot%202020-06-11%20at%2011.55.52%20AM.png?alt=media\&token=24dfca99-aa22-435d-a420-a80902855fd7)

#### **Technical Method**

* Tool Used: plots\_module.r
* Input:&#x20;
  * title\_file.txt
  * coverage\_agg.txt
  * average\_coverage\_across\_exon\_targets\_duplex\_A.txt
* Output: N/A

#### **Interpretation**

* Library input should be \~5-20ng for ctDNA, \~200ng for buffy coats (or the maximum amount available if these thresholds can’t be met)
* Capture input should be \~500ng or maximum available after library generation
* Expected range of coverage values:
  *
    * Raw coverage A panel:&#x20;
      * ctDNA: \~ 15000x-20000x
      * Buffy Coat: \~ 500x-1000x
    * Raw coverage B panel:&#x20;
      * ctDNA: \~ 1000x-1,500x
      * Buffy Coat: \~ 500x-1000x
    * Duplex coverage A panel:&#x20;
      * ctDNA: \~ 500x-2000x&#x20;
      * Buffy Coat: \~ 10x-50x

{% hint style="info" %}
**Note:** Samples that don’t meet the library input criteria will have lower coverage
{% endhint %}

\
&#x20;
